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Methadone Cassette Test
Drug Uses
Methadone Cassette Test a rapid, visual, qualitative, competitive binding immunoassay for the detection of Methadone and Methadone metabolites in human urine.
Methadone Cassette Test provides a fast confirmation of the presence of Methamphetamine in the subject sample at a minimum cut-off sensitivity level of 300 ng/ml.
How Taken
Allow test device, urine specimen, and/or controls to equilibrate to room temperature (15-30 degrees C) prior to testing.
- Bring the pouch to room temperature before opening it. Remove the test device from the sealed pouch and use it as soon as possible;
- Place the test device on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine to the specimen well of the test device, and then start the timer. Avoid trapping air bubbles in the specimen well;
- Wait for the red line to appear. The result should be read at 5 minutes. Results may be stable up to 4 hours after test initiation.
NEGATIVE: TWO COLOR BANDS - The appearance of two color bands within the result window indicates a negative test result. No Methadone above the cut-off level has been detected. The color of the Test band may be lighter or darker than that of the Control Band.
POSITIVE: ONE COLOR BAND - The appearance of only one color band within the result window indicates the result is positive, i.e. the specimen contains Methadone at a concentration above the cut-off level.
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test device. If the problem persists, discontinue using the lot immediately and contact your local distributor.
Drug Class and Mechanism
Methadone Cassette Test incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a Methadone derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device.
When Methadone levels are below 300 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone, producing a pink-rose colored band that indicates a negative result. Conversely, when Methadone levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex.
The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.
Missed Dose
You can use Methadone Cassette Test whenever you feel you need to do so.
Storage
Methadone Cassette Test is should be stored at room temperature (15 to 30 degrees C) for the duration of the shelf-life. Methadone Cassette Test must remain in the foil wrap or fog pouch until ready for use.
